کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236310 | 45143 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of β-galactosidase by Kluyveromyces marxianus under oscillating dissolved oxygen tension
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of β-galactosidase by Kluyveromyces marxianus under oscillating dissolved oxygen tension Production of β-galactosidase by Kluyveromyces marxianus under oscillating dissolved oxygen tension](/preview/png/10236310.png)
چکیده انگلیسی
The effect of oscillating dissolved oxygen tension (DOT) on the metabolism of Kluyveromyces marxianus was investigated, particularly on the productivity of the enzyme β-galactosidase or lactase (EC 3.2.1.23). Sinusoidal DOT oscillations were established by manipulating the inflowing gases (nitrogen and oxygen), at wave periods of 300, 600 and 1200 s and DOT ranging from 0 to 20%. Cultures carried out at different constant DOT ranging from 0 to 60% of air saturation were used for comparison. Oscillations at the shortest period tested (i.e., 300 s) resulted in higher specific and volumetric enzyme activity (2800 U/g and 31,700 U/l, respectively) and ethanol concentration (12.3 g/l) compared with cultures carried out at longer wave periods (600 and 1200 s) and those cultures carried out at constant DOT (0, 1, 5 and 20%). Final biomass concentration was not affected by oscillating DOT (12.2±0.7 g/l) and specific growth rate was slightly affected. These findings suggest that some non-ideal DOT control conditions can be advantageous for the case of lactase production, leading to increases in the enzyme volumetric or specific activity, without affecting maximum biomass concentration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 773-778
Journal: Process Biochemistry - Volume 40, Issue 2, February 2005, Pages 773-778
نویسندگان
Gabriela Cortés, Mauricio A. Trujillo-Roldán, Octavio T. RamıÌrez, Enrique Galindo,