کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10276151 | 463419 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical study by a redox probe of the chemical post-functionalization of N-substituted polypyrrole films: Application of a new approach to immobilization of biotinylated molecules
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Electrochemical study by a redox probe of the chemical post-functionalization of N-substituted polypyrrole films: Application of a new approach to immobilization of biotinylated molecules Electrochemical study by a redox probe of the chemical post-functionalization of N-substituted polypyrrole films: Application of a new approach to immobilization of biotinylated molecules](/preview/png/10276151.png)
چکیده انگلیسی
In order to study the post-polymerization functionalization of a poly(N-substituted pyrrole) film, the β-ferrocene ethylamine used as redox probe has been immobilized via a chemical coupling on the surface of a preformed polypyrrole film bearing activated ester groups. From the electrochemical response of the anchored ferrocene units, the post-polymerization functionalization efficiency has been evaluated to be 20-22%. This level is the same whatever the length of spacer arm (2, 5 and 12 carbons) linking the activated ester groups to the polypyrrole backbone and the hydrophilic and hydrophobic nature of this polymer. Using a similar strategy based on this chemical grafting, we have developed a new approach to the immobilization of biotin entities onto the ester activated-polypyrrole film surface. From fluorescence measurements, it appears that the anchoring of streptavidine bearing a fluorophore (streptavidin-R-phycoerythrin, SAPE) on this biotinylated polypyrrole film gives a fluorescence intensity similar to the one achieved from the immobilization of SAPE onto a film electrosynthesized from pyrrole monomers bearing biotin units. Thus, this result shows the efficiency of this new functionalization method for attaching biotinylated entities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 578, Issue 2, 1 May 2005, Pages 301-313
Journal: Journal of Electroanalytical Chemistry - Volume 578, Issue 2, 1 May 2005, Pages 301-313
نویسندگان
M.-L. Calvo-Muñoz, B. Ehui-Avo Bile, M. Billon, G. Bidan,