Sensitization of sexual behaviors in ovariectomized Long-Evans rats is induced by a subthreshold dose of estradiol benzoate and attenuated by repeated copulation

Ovariectomy (OVX) abolishes the expression sexual behaviors in the rat, but they can be fully reinstated by sequential administration of estradiol benzoate (EB) followed by progesterone (P). When administered alone, 5 or 10 μg EB (but not 2 μg) acutely induce only low levels of lordosis, whereas repeated administration potentiates lordosis and induces sexually appetitive behaviors (e.g., hops, darts, solicitations, ear wiggles). The mechanisms mediating this behavioral sensitization are poorly understood, and it is not clear whether stimulation from the male during repeated copulation plays a role. OVX Long-Evans rats were given 4 sexual training sessions with EB (10 μg) and P (500 μg) 48 and 4 h prior to testing, respectively, in a unilevel 4-hole pacing chamber followed by a 2-week hormone washout. Females were then treated with 2 μg or 10 μg EB 48 h prior to copulation on Tests 1 and 8. On Tests 2-7, a group of females was treated with 10 μg EB and allowed to copulate with a male (10 μgEB/Male, n = 16), or treated with 2 μg or 10 μg EB and placed in the chamber alone (2 μgEB/Alone, n = 6; 10 μgEB/Alone, n = 18). A negative control group was treated with the oil vehicle and placed in the chamber alone (Oil/Alone, n = 6) on Tests 2-7, but treated with 2 μg EB prior to copulatory Tests 1 and 8. All groups, except Oil, displayed behavioral sensitization to EB, suggesting that repeated administration EB is both necessary and sufficient to induce sensitization. Appetitive behaviors were attenuated in those that copulated on every session. Pacing was disrupted in all groups. Together these results suggest that EB activates excitatory mechanisms to promote the expression of sexual behaviors, which are potentiated across time under certain conditions. In contrast, copulatory stimulation attenuates behavioral sensitization to EB.