کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10306688 | 547424 | 2013 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regional distribution of 5α-reductase type 2 in the adult rat brain: An immunohistochemical analysis
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کلمات کلیدی
stratum oriensAOLPMCoVPMDLGVMHCLiHYPLPLVTAHDBPAR1VPLMe5ventral lateral thalamic nucleusGlobus pallidumACCMePVCA1–CA3CPUSTHBLAmitral cell layer of olfactory bulbLaDLSNCENTOPTSNRPNCDen5α-reductase - 5α-ردوکتازAcb - ACBMHb - MHBMnR - MNRneurosteroids - Neurosteroidsstratum radiatum - stratat radiatumSuG - SUGandrogens - آندروژنImmunohistochemistry - ایمونوهیستوشیمیlateral ventricle - بطن جانبی3rd ventricle - بطن سوم4th ventricle - بطن چهارمPir - بیش از حدThalamus - تالاموسsubstantia nigra - توده سیاهoptic tract - دستگاه نوریSubiculum - زیرکیولومPurkinje cells - سلول های Purkinjecerebral peduncle - سیب زمینی مغزیdentate gyrus - شکنج دندانه دارOcc - عجبfornix - فورنیکسOccipital cortex - قشر OccipitalEntorhinal cortex - قشر انتورینالfrontal cortex - قشر جلوییpiriform cortex - قشر پریکومArc - قوسgranular layer - لایه دانه ایpyramidal cell layer - لایه سلول هرمmolecular layer - لایه مولکولیlocus coeruleus - لوکوس سیرولئوسSubstantia nigra compacta - متراکم توده سیاهCerebellum - مخچهBrain - مغزTenia tecta - من لمس کردمventral tegmental area - ناحیه تگمنتوم شکمیaccumbens nucleus - هسته accumbensanterior olfactory nucleus - هسته بویایی قدامیposterior thalamic nucleus - هسته تالامال خلفیVentral posteromedial thalamic nucleus - هسته تالاموم پس از معدهreticular thalamic nucleus - هسته تالامیک رتیکالventral posterolateral thalamic nucleus - هسته تالامیک پست سلولی شکمیmedian raphe nucleus - هسته رافائل مرکزیdorsal raphe nucleus - هسته رافهMesencephalic trigeminal nucleus - هسته سه هسته ای Mesencephalicmedial habenular nucleus - هسته عصب مرکزیposteromedial cortical amygdaloid nucleus - هسته قارچی عصب مرکزیLateral amygdaloid nucleus - هسته مرکزی آمیگدولید جانبیdorsal lateral geniculate nucleus - هسته منشاء جانبی پشتی پشتیarcuate hypothalamic nucleus - هسته هیپوتالاموس قوس الکتریکیPontine nuclei - هسته پونتینmedial geniculate nucleus - هسته ژنومیک محیطیdorsal endopiriform nucleus - هسته ی endopiriform پشتیSubthalamic nucleus - هسته ی زیرهالامیکHypothalamus - هیپوتالاموسanterior hypothalamus - هیپوتالاموس قدامیHippocampus - هیپوکامپ Caudate putamen - پاتامن Caudatecingulate cortex - کورتکس کانگولتInferior colliculus - کولیکولوس پایینAON - یکم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regional distribution of 5α-reductase type 2 in the adult rat brain: An immunohistochemical analysis Regional distribution of 5α-reductase type 2 in the adult rat brain: An immunohistochemical analysis](/preview/png/10306688.png)
چکیده انگلیسی
The enzyme 5α-reductase (5αR) catalyzes the conversion of testosterone and other Î4-3-ketosteroids into their 5α-reduced metabolites. Of the five members of the 5αR family, the type 2 enzyme (5αR2) plays a key role in androgen metabolism, and is abundantly distributed in the urogenital system. Although 5αR2 has been reported to be highly expressed in the brain during early developmental stages, little is currently known on its anatomical and cellular distribution in the adult brain. Thus, the present study was designed to determine the detailed localization of 5αR2 in the adult rat brain, using a highly specific polyclonal antibody against this isoform. Parasagittal and coronal sections revealed 5αR2 immunoreactivity throughout most brain regions, with strong immunolabeling in the layers III and VI of the prefrontal and somatosensory cortex, olfactory bulb, thalamic nuclei, CA3 field of hippocampus, basolateral amygdala and Purkinje cell layer of cerebellum. Lower 5αR2 levels were detected in the hypothalamus and midbrain. Moreover, double labeling fluorescence with confocal laser scanning microscopy (CLSM) revealed that 5αR2 is localized in neurons, but not in glial cells. Specifically, the enzyme was documented in the pyramidal neurons of the cortex by CLSM analysis of simultaneous Golgi-Cox and immunofluorescent staining. Finally, low levels of 5αR2 expression were identified in GABAergic cells across the cortex, hippocampus and striatum. These findings show that, in the adult brain, 5αR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Psychoneuroendocrinology - Volume 38, Issue 2, February 2013, Pages 281-293
Journal: Psychoneuroendocrinology - Volume 38, Issue 2, February 2013, Pages 281-293
نویسندگان
M. Paola Castelli, Alberto Casti, Angelo Casu, Roberto Frau, Marco Bortolato, Saturnino Spiga, Maria Grazia Ennas,