کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10375011 | 880028 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of gemini surfactants butane-1,4-diyl-bis(alkyldimethylammonium bromide) with DNA
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
The size and structure of aggregates formed by interaction of DNA with homologous series of cationic gemini surfactants butane-1,4-diyl-bis(alkyldimethylammonium bromide) (CnGS, n = 10-16 is the number of alkyl carbons) were investigated using UV-vis turbidity, dynamic light scattering and small-angle synchrotron X-ray (SAX) diffraction. The detailed analysis of turbidity in the range of λ = 450-600 nm indicates an anomaly in the growth of CnGS + DNA aggregates with increasing concentration of CnGS, possibly involving changes of structure and size of aggregates. Using dynamic light scattering, changes of the effective diameter of CnGS + DNA (n = 12 and 16) aggregates formed in the CnGS concentration range 0.002-0.140 mmol/l were observed. SAX diffractograms show the presence of long-range organization of CnGS + DNA (n = 12, 13, 14 and 16) aggregates due to DNA interaction with CnGS above the critical micellar concentration. The CnGS + DNA (n = 12, 13 and 14) aggregates at 25 °C are packed in a lattice of two-dimensional hexagonal symmetry. With increasing C14GS:DNA molar ratio the changes of the lattice parameter in the range of 4.80-5.27 nm are observed at 25 °C. The aggregates undergo structural changes induced by temperature in the range 60-95 °C, which are accompanied by changes of the diffraction patterns, namely in the region of reciprocal spacing s = 0.15-0.30 nmâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 42, Issue 1, 25 April 2005, Pages 59-68
Journal: Colloids and Surfaces B: Biointerfaces - Volume 42, Issue 1, 25 April 2005, Pages 59-68
نویسندگان
Daniela UhrÃková, Igor Zajac, Martina DubniÄková, Martin PisárÄik, Sérgio S. Funari, Gert Rapp, Pavol Balgavý,