کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10385101 | 882486 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Parameters optimization of recombinant hepatitis B antigen adsorption-desorption in purification process on three different kinds of diatomaceous earth matrix
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Parameters optimization of recombinant hepatitis B antigen adsorption-desorption in purification process on three different kinds of diatomaceous earth matrix Parameters optimization of recombinant hepatitis B antigen adsorption-desorption in purification process on three different kinds of diatomaceous earth matrix](/preview/png/10385101.png)
چکیده انگلیسی
Maximum adsorbed/desorbed efficiency for Celite-A, Celite-B and Celite-C were obtained in optimum parameters, 78%, 45% and 88% respectively. Batch shaking experiments were performed to examine the effects of initial solution pH, agitation speed, contact time and initial antigen concentrations. The maximum adsorption capacity for hepatitis B antigen was obtained 66.2 μg gâ1, 32.4 μg gâ1 and 72.6 μg gâ1 for Celite-A, Celite-B and Celite-C in case of experimental optimal conditions, respectively. The results indicated that Langmuir isotherm provide the best correlation of experimental data for Celite-A and Celite-C, while the Freundlich isotherm better fitted to experimental data for Celite-B. Results also indicated antigen adsorption onto the celite is best represented with a pseudo second-order kinetic model. Surface characterization was performed by FTIR and FESEM analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Research and Design - Volume 92, Issue 11, November 2014, Pages 2782-2791
Journal: Chemical Engineering Research and Design - Volume 92, Issue 11, November 2014, Pages 2782-2791
نویسندگان
Parisa Ghaisari, Seyed Nezamedin Hosseini, Shahram Sharifnia, Maryam Khatami,