کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1038580 | 944227 | 2009 | 5 صفحه PDF | دانلود رایگان |
Marble monuments and facades are susceptible to microbial colonization. Microbial growth on a marble surface can develop into unsightly red stains whose removal has proven problematic. The purpose of this study was to determine if the red-brown stains on Isamu Noguchi's marble sculpture Slide Mantra (1991) could be caused by pigment-producing microorganisms and to assess the potential of enzymatic stain remediation. Traditional cell culture methods were used to isolate a pigmented bacterium from a stained area of the sculpture. Sequencing and analysis of the 16S rRNA gene identified the organism as a strain of Serratia marcescens, and FT-IR spectroscopy demonstrated that the pigment produced by the bacteria was most likely a prodigiosin. Decolorization of the pigment in solution demonstrated that the enzyme laccase from the fungus Trametes versicolor has potential as a decolorizing agent. This study suggests that enzymatic decolorization may be applicable to stains on culturally significant marble caused by microbial colonization.
Journal: Journal of Cultural Heritage - Volume 10, Issue 3, July–September 2009, Pages 362–366