Novel organic solvent-responsive expression vectors for biocatalysis: Application for development of an organic solvent-tolerant biodesulfurizing strain

► We constructed two organic solvent-responsive expression vectors. ► We constructed an organic solvent-tolerant biodesulfurizing strain Pseudomonas putida DS23. ► We examined the biodesulfurizing activity of P. putida DS23. ► Strain DS23 was better than the strain constructed with IPTG-induced vector.