Anaerobic biohydrogen production from wheat stalk by mixed microflora: Kinetic model and particle size influence

► Cumulative production of H2, HAc and HBu decreased with particle size increasing. ► A modified Gompertz equation described cumulative production of H2, HAc and HBu well. ► Main product formation was associated with cellulose and hemicellulose degradation. ► Particle size showed important influence on anaerobic H2 production from wheat stalk.