Optimization of the biomass production of oil algae Chlorella minutissima UTEX2341

► Glycerin and casein were the best for algae UTEX2341 biomass and lipid production. ► Biomass and lipid yields were 28.7 and 11.9 times higher than that by Illman and Oh. ► The unsaturated fatty acids were the main parts of FAME. ► 94% of the fatty acid was composed of the palmitic, oleic, linoleic and γ linoleic.