Overproduction of alkaline polygalacturonate lyase in recombinant Escherichia coli by a two-stage glycerol feeding approach

► Overproduction of alkaline polygalacturonate lyase (PGL) by recombinant Escherichia coli. ► A two-stage glycerol feeding approach was developed to enhance PGL yield. ► Under the optimal conditions, the total alkaline PGL yield reached 371.86 U mL−1.