Ethanol production from the enzymatic hydrolysis of non-detoxified steam-exploded corn stalk

► An adapted strain of Pichia stipitis was used to produce ethanol from corn stalk. ► The enzymatic hydrolysate from steam-exploded corn stalk un-detoxified was fermented. ► The ethanol concentration of 43.42 g/L and yield of 0.47 gs/gp were reached. ► The strain reduced water consumption and lowered equipment investment in the process. ► Referring cost and technology can be greatly reduced and simplify.