Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01

► We isolate a moderate halophile Halomonas TD01 from environmental samples. ► This strain could reach high cell density containing amounts of PHA in MM-G medium. ► An open process without sterilization is feasible based on Halomonas TD01. ► An open and continuous fermentation process has been developed.