Pd nanoparticle assemblies-As the substitute of HRP, in their biosensing applications for H2O2 and glucose

► The spherical porous Pd nanostructures have been synthesized by a simple method. ► Such Pd can promote the electron transfer and catalyze the reduction of H2O2. ► They are used as horseradish peroxidase substitutes in electron transfer process. ► Based on the Pd and glucose oxidase, a sensitive glucose biosensor is developed. ► This biosensor provides a strategy for early diagnosis and prevention of diabetes.