Paper-based chemiluminescence ELISA: Lab-on-paper based on chitosan modified paper device and wax-screen-printing

► Sandwich CL-ELISA was introduced onto μPADs to perform high-sensitivity and high-selectivity POCT. ► Chitosan was used to modify μPADs to covalently immobilize antibodies on μPADs to realize high-performance POCT. ► Wax-screen-printing was demonstrated for further development of this technique in simple and low-cost application.