کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429458 | 909794 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive sandwich electrochemical immunosensor for alpha fetoprotein based on prussian blue modified hydroxyapatite
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Sensitive sandwich electrochemical immunosensor for alpha fetoprotein based on prussian blue modified hydroxyapatite Sensitive sandwich electrochemical immunosensor for alpha fetoprotein based on prussian blue modified hydroxyapatite](/preview/png/10429458.png)
چکیده انگلیسی
A sandwich electrochemical immunosensor for the sensitive determination of alpha fetoprotein (AFP) has been fabricated. Prussian blue modified hydroxyapatite (PB@HAP) was firstly prepared and used as electrochemical label due to the wonderful conductivity and good biocompatibility of HAP. The results proved that the immunosensor fabricated using the label based on PB@HAP loaded with horse radish peroxidase (HRP) and secondary anti-AFP antibody (Ab2) (PB@HAP-HRP-Ab2) had high sensitivity, and the sensitivity of the label PB@HAP-HRP-Ab2 was much higher than labels of PB@HAP-Ab2, PB-HRP-Ab2 and HAP-HRP-Ab2. The mixture of graphene sheet (GS) and thionine (TH) was not only used to immobilize anti-AFP antibody (Ab1) but also took part in the signal amplification. The amperometric signal increased linearly with AFP concentration in the range of 0.02-8Â ng/mL with a low detection limit of 9Â pg/mL. The immunosensor had the advantages of high sensitivity, good selectivity and good stability, and was applied to the analysis of AFP in serum sample with satisfactory results. Due to the low-cost and easy synthesis of PB@HAP, the screen-printed electrodes could be used instead of the bare glass carbon electrode in order to achieve mass production. In addition, it had potential application in the detection of other tumor markers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 112-116
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 112-116
نویسندگان
Yuxue Dai, Yanyan Cai, Yanfang Zhao, Dan Wu, Bao Liu, Ru Li, Minghui Yang, Qin Wei, Bin Du, He Li,