کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429461 | 909794 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNAzyme-based highly sensitive electronic detection of lead via quantum dot-assembled amplification labels
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An electronic DNAzyme sensor for highly sensitive detection of Pb2+ is demonstrated by coupling the significant signal enhancement of the layer-by-layer (LBL) assembled quantum dots (QDs) with Pb2+ specific DNAzymes. The presence of Pb2+ cleaves the DNAzymes and releases the biotin-modified fragments, which further hybridize with the complementary strands immobilized on the gold substrate. The streptavidin-coated, QD LBL assembled nanocomposites were captured on the gold substrate through biotin-streptavidin interactions. Subsequent electrochemical signals of the captured QDs upon acid dissolution provide quantitative information on the concentrations of Pb2+ with a dynamic range from 1 to 1000Â nM. Due to the dramatic signal amplification by the numerous QDs, subnanomolar level (0.6Â nM) of Pb2+ can be detected. The proposed sensor also shows good selectivity against other divalent metal ions and thus holds great potential for the construction of general DNAzyme-based sensing platform for the monitoring of other heavy metal ions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 135-138
Journal: Biosensors and Bioelectronics - Volume 28, Issue 1, 15 October 2011, Pages 135-138
نویسندگان
Haixia Zhang, Bingying Jiang, Yun Xiang, Jiao Su, Yaqin Chai, Ruo Yuan,