کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429541 | 909796 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme integrated silicate-Pt nanoparticle architecture: A versatile biosensing platform
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enzyme integrated silicate-Pt nanoparticle architecture: A versatile biosensing platform Enzyme integrated silicate-Pt nanoparticle architecture: A versatile biosensing platform](/preview/png/10429541.png)
چکیده انگلیسی
A novel 3-D nanoarchitectured platform based on Pt nanoparticles (nPts) is developed for the sensing of sub-nanomolar levels of hydrogen peroxide and for the fabrication of amperometric biosensor for uric acid, cholesterol and glucose. The nPts have been immobilized on the thiol functional group containing sol-gel silicate 3-D network derived from 3-mercaptopropyltrimethoxysilane (MPTS). The nanoparticles on the 3-D architecture have size distribution between 7 and 10Â nm. The nPts on the platform efficiently catalyze the oxidation of H2O2 at the potential of +0.45Â V in the absence of enzymes and redox mediators. This nanoarchitectured platform is highly sensitive and can detect H2O2 at sub-nanomolar levels (0.1Â nM) in neutral solution. The nanoarchitectured platform does not suffer from interference due to other common easily oxidizable interfering agents. Excellent reproducibility, long-term storage and operational stability are observed. This platform is used to determine H2O2 concentration in rainwater and for the fabrication of biosensors. Amperometric oxidase-based biosensing platforms are developed by integrating the enzymes and nPts with the silicate network for the sensing of uric acid cholesterol and glucose. The enzyme encapsulated 3-D architecture retains the enzymatic activity and efficiently detects enzymatically generated H2O2 without any interference. These biosensors are stable and show excellent sensitivity and fast response time. A linear response was obtained for a wide concentration range of all analytes. The practical utilization of the biosensor for the measurement of uric acid, cholesterol and glucose in serum sample is demonstrated. The biological sample analysis was validated with clinical laboratory measurements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 2960-2966
Journal: Biosensors and Bioelectronics - Volume 26, Issue 6, 15 February 2011, Pages 2960-2966
نویسندگان
Bikash Kumar Jena, C. Retna Raj,