کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429590 | 909845 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Point of care testing system via enzymatic method for the rapid, efficient assay of glycated albumin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
The ratio of glycated albumin to albumin concentration in serum is termed the glycated albumin (GA) value. The GA value provides a time-averaged index of the state of glycemic control for the previous 2 weeks. In this study, a dry chemistry system (GA monitor) via an enzymatic method was proposed in order to provide a GA value measurement for point of care testing (POCT). The GA monitor was made from three devices a set of test-tapes, a test-strip and an optical analyzer. A GA test-tape, a ketoamine test-tape and an albumin test-tape were enclosed in the fabricated test-strip. Time-course changes of the optical characteristics were evaluated using the test-strip. It was found that the three test tapes must be enclosed in the test-strip to create a dry chemistry system for small sample volumes (20 μl). A temperature control unit, which could hold the temperature of the GA test-tape at 45 °C and at 25 °C for the other two types of test-tape was incorporated into the optical analyzer. With the GA test-tape held separately and controlled at 45 °C, the analytical time decreased to one-third of the time taken for the three tapes at 25 °C. The analytical accuracy of the three types of test-tape showed favorable results, with R2 values of 0.96-0.98 and coefficients of variation (CV) of 2.4-6.8%. Compared with a commercially available liquid chemistry system, the analytical accuracy of the GA monitor exhibited a relatively favorable linearity of R = 0.82. According to these results, a new GA value analytical system was realized, in which the GA value could be assayed within five minutes using only 20 μl of blood sample with a disposable test-strip. This system could potentially be used for clinical purposes as test equipment for rapid and efficient POCT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 426-432
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 426-432
نویسندگان
Masaki Yamaguchi, Shigenori Kambe, Takashi Eto, Masaru Yamakoshi, Takuji Kouzuma, Nobuyuki Suzuki,