کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429595 | 909845 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers](/preview/png/10429595.png)
چکیده انگلیسی
Composite self-excited PZT-glass cantilevers (5 and 3Â mm in length, 1.8 and 2.0Â mm wide) were fabricated and their resonance characteristics were determined in air and at 1Â mm liquid immersion. In air, resonance occurred at 65.8 and 63.4Â kHz for the two cantilevers used in this paper. Monoclonal antibody (MAb) specific to the pathogen Escherichia coli (E. coli) O157:H7 was immobilized at the cantilever glass tip, and then exposed to pathogen in the concentration range of 7Â ÃÂ 102 to 7Â ÃÂ 107Â bacteria/mL. Resonance of the second mode decreased due to pathogen attachment in accordance with a proposed kinetic model. The specific attachment rate constant was found to be 3Â ÃÂ 10â9 to 5Â ÃÂ 10â9Â minâ1Â (cell/mL)â1. Exposure to a mixed population containing both a pathogenic and non-pathogenic strain showed that the antibody-immobilized cantilever is highly selective, thus demonstrating its usefulness for detecting water-borne pathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 462-473
Journal: Biosensors and Bioelectronics - Volume 21, Issue 3, 15 September 2005, Pages 462-473
نویسندگان
Gossett A. Campbell, Raj Mutharasan,