An improved coating for the isolation and quantitation of interferon-γ in spiked plasma using surface plasmon resonance (SPR)

From the coatings tested, the non-modified dextran-coated SPR sensor disks prove to be best suited for the detection of IFN-γ in complex matrices like plasma. The interaction of IFN-γ in both diluted plasma and buffer is comparable and concentrations of IFN-γ of 250 ng ml−1 and higher can be detected in both buffer and 100×-diluted plasma. The non-specific adsorption of plasma components is low, whereas the specific IFN-γ response is relatively high.