کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429680 | 909858 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection and quantification of proteins using self-excited PZT-glass millimeter-sized cantilever
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A composite self-excited PZT-glass cantilever (4 mm in length and 2 mm wide) was fabricated and used to measure the binding and unbinding of model proteins. A key feature of the cantilever is that its resonant frequency is dependent on its mass. The fabricated cantilever has mass change sensitivity in liquid of 7.2 Ã 10â11 g/Hz. Resonant frequency change was measured as protein reacted or bound with the sensing glass cantilever surface. Protein concentrations, 0.1 and 1.0 mg/mL, which resulted in nanogram mass change were successfully detected. The mass change sensitivity gave a total mass change of 54 ± 0.45 ng for the binding of anti-rabbit IgG (biotin conjugated) to rabbit IgG immobilized cantilever and the subsequent binding of captavidin. The unbinding of anti-rabbit IgG and captavidin gave a total mass change of 54 ± 1.70 ng. Fluorescence based assays showed the combined mass of both proteins in the released samples was 54 ± 2.24 ng. The binding kinetics of the model proteins is modeled as first order. The initial binding rate constant of anti-rabbit IgG to rabbit IgG was 1.36 ± 0.02 (min (mg/mL))â1. The initial binding rate constant of captavidin to biotinylated anti-rabbit IgG was (2.57 Ã 10â1) ± 0.003 (min (mg/mL))â1. The significance of the results we report here is that millimeter-sized PZT-actuated glass cantilevers have the sensitivity to measure in real-time protein-protein binding, and the binding rate constant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 21, Issue 4, 15 October 2005, Pages 597-607
Journal: Biosensors and Bioelectronics - Volume 21, Issue 4, 15 October 2005, Pages 597-607
نویسندگان
Gossett A. Campbell, Raj Mutharasan,