کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10431404 910213 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Embryonic stem cell-derived osteocytes are capable of responding to mechanical oscillatory hydrostatic pressure
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
پیش نمایش صفحه اول مقاله
Embryonic stem cell-derived osteocytes are capable of responding to mechanical oscillatory hydrostatic pressure
چکیده انگلیسی
Here, we show that ESC-osteoblastogenesis is followed by the generation of osteocytes and then mechanically load ESC-derived osteogenic cultures in a compression chamber using a cyclic loading protocol. Following mechanical loading of the cells, iNOS mRNA was upregulated 31-fold, which was consistent with a role for iNOS as an immediate early mechanoresponsive gene. Further analysis of matrix and bone-specific genes suggested a cellular response in favor of matrix remodeling. Immediate iNOS upregulation also correlated with a concomitant increase in Ctnnb1 and Tcf7l2 mRNAs along with increased nuclear TCF transcriptional activity, while the mRNA for the repressive Tcf7l1 was downregulated, providing a possible mechanistic explanation for the noted matrix remodeling. We conclude that ESC-derived osteocytes are capable of responding to relevant mechanical cues, at least such that mimic oscillatory compression stress, which not only provides new basic understanding, but also information that likely will be important for their use in cell-based regenerative therapies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 48, Issue 10, 16 July 2015, Pages 1915-1921
نویسندگان
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