کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10435396 910922 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
پیش نمایش صفحه اول مقاله
The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts
چکیده انگلیسی
Due to the limited number of cells available in endothelial cell (EC) seeding of small diameter vascular grafts, high seeding rate and ideal proliferation are normally required and can be achieved by optimizing the EC seeding and culture procedures. In this study, by using rotational seeding at 0.16 rpm for 12 h in an incubator, 90% cells were successfully seeded on the polyurethane vascular grafts. Following a period of 72 h of static culture, the cell retention after 6 h of flushing could reach 90%. The retention was further enhanced after perfuse culture (9 cm/s). The optimal procedures to prepare a polyurethane vascular graft (4-mm i.d., 4 cm long) populated with firmly attached EC were therefore: (1) seeding the graft with 0.5 ml of cell suspension containing ∼105 cells rotated at 0.16 rpm for 12 h; (2) culturing the seeded graft in static for 72 h; and (3) culturing the graft by perfusion (9 cm/s) for another 72 h to 7 days. These procedures consistently resulted in a graft covered with confluent vein EC that fully retained on the surface after 6 h of in vitro flushing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Medical Engineering & Physics - Volume 27, Issue 3, April 2005, Pages 267-272
نویسندگان
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