کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10435396 | 910922 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Due to the limited number of cells available in endothelial cell (EC) seeding of small diameter vascular grafts, high seeding rate and ideal proliferation are normally required and can be achieved by optimizing the EC seeding and culture procedures. In this study, by using rotational seeding at 0.16Â rpm for 12Â h in an incubator, 90% cells were successfully seeded on the polyurethane vascular grafts. Following a period of 72Â h of static culture, the cell retention after 6Â h of flushing could reach 90%. The retention was further enhanced after perfuse culture (9Â cm/s). The optimal procedures to prepare a polyurethane vascular graft (4-mm i.d., 4Â cm long) populated with firmly attached EC were therefore: (1) seeding the graft with 0.5Â ml of cell suspension containing â¼105 cells rotated at 0.16Â rpm for 12Â h; (2) culturing the seeded graft in static for 72Â h; and (3) culturing the graft by perfusion (9Â cm/s) for another 72Â h to 7 days. These procedures consistently resulted in a graft covered with confluent vein EC that fully retained on the surface after 6Â h of in vitro flushing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Medical Engineering & Physics - Volume 27, Issue 3, April 2005, Pages 267-272
Journal: Medical Engineering & Physics - Volume 27, Issue 3, April 2005, Pages 267-272
نویسندگان
Shan-hui Hsu, I-jine Tsai, Da-jun Lin, David C. Chen,