Chitosan polyplex mediated delivery of miRNA-124 reduces activation of microglial cells in vitro and in rat models of spinal cord injury

A chitosan/miRNA-124 nanoparticle was formed and shown to transfect and reduce cytotoxic molecules and activation in classically activated ex vivo microglia. In vivo transfection was seen to be specific to macrophages/microglia in spinal microinjections, and subsequently reduced their activation in microinjection and hemisection models of spinal cord injury.320