Nanostructured electrochemical biosensor for th0065 detection of the weak binding between the dengue virus and the CLEC5A receptor

An effective method for detecting weak molecular bonding between the dengue virus (DV) and its receptor C-type lectin domain family 5, member A (CLEC5A) has been developed. Label-free detections of the ultra weak binding were carried out via a highly sensitive nanostructured sensing electrode with gold nanoparticles (GNPs) uniformly deposited on a nanohemisphere array. Experimental results demonstrate that the proposed approach is a highly promising method for investigating weak molecular interactions such as the ligand-receptor interaction of DV and enterovirus.455