Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Schematic diagram depicting the production and culture of complex 3D multicellular constructs using a sequential cell seeding procedure. Astrocytes (1) were seeded on aligned nanofiber-collagen hydrogel constructs followed by addition of oligodendrocyte precursor cells [OPCs; (2)] after astrocyte alignment. The multicellularity of the constructs containing OPCs, mature oligodendrocytes, type I and type II astrocytes is shown in (3).145