NMR for direct determination of Km and Vmax of enzyme reactions based on the Lambert W function-analysis of progress curves

► Direct determination of Km and Vmax from progress curves using 1H NMR spectroscopy. ► Applying Lambert W function analysis for fitting the experimental progress curves. ► NMR can easily detect and quantify in situ all reaction products and substrates. ► NMR allows the direct analysis of the stereochemistry of the products. ► NMR is a powerful tool to analyze Michaelis-Menten kinetics of enzyme reactions.