Characterization of the single-stranded DNA binding protein pVVGJΦ of VGJΦ phage from Vibrio cholerae

► pVVGJΦ a DNA binding protein of phage VGJΦ has a general affinity for ssDNA. ► SEC revealed a major species of 27.0 kDa, suggesting a homodimeric protein. ► pVVGJΦ binds ssDNA at extreme temperatures, stable after extended times. ► After a year at -20 °C the protein kept its biological activity and oligomericity. ► pVVGJΦ is interesting and attractive for further application in biotechnology.