کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10537324 | 962714 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pressure-tuning FT-IR spectroscopic study on the helix-coil transition of Ala-rich oligopeptide in aqueous solution
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We investigated the effect of pressure on the helix-coil transition of an Ala-rich peptide (AK16: YGAAKAAAAKAAAAKA-NH2) in aqueous solution by FT-IR spectroscopy. The spectra of the amide I' region of AK16 in aqueous solution was decomposed into some component bands using a curve fitting method. The peak at around 1635 cm â1 corresponding to the solvent exposed α-helix conformer increases with increasing pressures, while the peak at around 1655 cm â1 corresponding to the random coil conformer decreases. From the pressure dependence of the band intensities, we determined the volume change from the α-helix to random coil conformers of AK16 to be + 10.5 ± 0.3 cm3/mol. The positive volume change is different from the negative volume change generally observed in the pressure denaturation of proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1750, Issue 1, 15 June 2005, Pages 1-4
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1750, Issue 1, 15 June 2005, Pages 1-4
نویسندگان
Takahiro Takekiyo, Akio Shimizu, Minoru Kato, Yoshihiro Taniguchi,