کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10537925 | 962882 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and crystallization of human DPY-30-like protein, an essential component of dosage compensation complex
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Human DPY-30-like is a homolog of C. elegans DPY-30. DPY-30 is an essential component of dosage compensation machinery and loss of dpy-30 activity results in XX-specific lethality. In XO animals, DPY-30 is required for developmental processes other than dosage compensation. In yeast, the homolog of DPY-30, Saf19p, functions as a member of histone 3 lysine 4 methylation complex, which is the key part of epigenetic developmental control. In this report, human DPY-30-like protein was overexpressed and purified with the goal of structure determination. It was crystallized at 291 K in hanging drops by the vapour diffusion technique from a precipitant solution consisting of (NH4)2SO4 (1.5-2.0Â M), Tris-HCl (0.1 M, pH 8.0). The crystal diffracted to 2.7 Ã
resolution at 100 K in-house and belongs to the space group P41212 or P43212 with unit-cell parameters of a = b = 74.5 Ã
, c = 87.0 Ã
, α = β = γ = 90.0°. The asymmetric unit contains two molecules with 49% solvent content. We also analyzed its biochemical and biophysical characterizations. Efforts are now under way to determine the molecular structure of the DPY-30-like. These studies will open a new avenue towards the structureâbased functional analysis of human DPY-30-like and dosage compensation machinery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1753, Issue 2, 1 December 2005, Pages 257-262
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1753, Issue 2, 1 December 2005, Pages 257-262
نویسندگان
Xiuhua Dong, Yong Peng, Ying Peng, Feng Xu, Xiaojing He, Feng Wang, Xiaozhong Peng, Boqin Qiang, Jiangang Yuan, Zihe Rao,