Production of the α-glycosidase inhibitor 1-deoxynojirimycin from Bacillus species

► Confirmation of microbial 1-deoxynojirimycin (DNJ) production by HPLC techniques. ► Identification of additional Bacillus strains that produce DNJ in large quantities. ► Importance of sorbitol supplementation on the yield of DNJ and precursors. ► These findings suggest a method for microbial mass production of DNJ.