Simultaneous detection of flavonoids and phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii using liquid chromatography tandem mass spectrometry

► Lysimachiae and Desmodii Styracifolii were firstly differentiated by LC-MS/MS. ► Orthogonal array design could be efficient to optimization of extraction method. ► Hierarchical cluster analysis and multiple graphs were successfully applied.