Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China

► The glucosinolates of broccoli germplasms from China in broccoli florets were investigated. ► The level of progoitrin in all commercial cultivars was comparatively high. ► The variations of individual glucosinolates among broccoli germplasms were significant. ► Specific glucosinolate lines and high-glucoraphanin lines were selected, respectively.