Anticoagulant activities of goby muscle protein hydrolysates

► Anticoagulant activities of protein hydrolysates prepared from goby muscle were investigated. ► The hydrolysate generated by the crude enzyme from Bacillus licheniformis NH1 displayed the highest anticoagulant activity. ► This hydrolysate was then fractionated by size exclusion chromatography on a Sephadex G-25 column into five major fractions. ► Four novel anticoagulant peptides, Leu-Cys-Arg, His-Cys-Phe, Cys-Leu-Cys-Arg and Leu-Cys-Arg-Arg, were identified.