Amperometric detection of ascorbic acid in honey using ascorbate oxidase immobilised on amberlite IRA-743

► We describe a differential amperometric determination of ascorbic acid in honey. ► Association FIA and a tubular reactor containing the ascorbate oxidase immobilised. ► We use a gold electrode modified by electrodeposition with palladium. ► The procedure adopted to immobilise the ascorbate oxidase enzyme was quick and simple.