High resolution melting analysis as a new approach to detect almond DNA encoding for Pru du 5 allergen in foods

► Development of a novel method to detect almond allergens in food. ► A real-time PCR assay targeting Pru du 5 gene was proposed. ► EvaGreen dye coupled to HRM analysis was used to increase specificity. ► High specific and sensitive detection of almond down to the level of 0.005% (w/w). ► HRM analysis can provide a powerful tool for the identification of food allergens.