کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10539790 | 963395 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of feruloylated lysophospholipid in a selected organic solvent medium
ترجمه فارسی عنوان
سنتز آنزیمی از لیفوسفولیپید فرولیلیتی در محیط حلال آلی انتخاب شده
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Feruloylated lysophospholipids (FLPs) were firstly synthesized from phosphatidylcholine (PC) and ethyl ferulate (EF) using lipase-catalysed interesterification in selected solvents at controlled water content. Kinds of lipases and single solvents were screened. Novozym 435 and toluene were found to be the suitable biocatalyst and solvent, respectively. Then tert-butanol, n-butanol, chloroform, isopropanol, acetone and DMSO were respectively added into toluene in order to increase conversion of products. The results showed that toluene/chloroform could significantly increase the conversion and the optimal combination of toluene and chloroform was 90:10 (v/v). The optimal conditions generated for FLPs production were a substrate molar ratio of 5:1 (PC/EF), a PC's hydrolytic time of 1.5Â h, an enzyme load of 60Â mg/ml, a solvent dosage of 5Â ml and a molecular sieves concentration (4Â Ã
) of 100Â mg/ml. Under these conditions, 40.51% of EF can be converted to FLPs, which were identified by TLC and HPLC-MS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 141, Issue 4, 15 December 2013, Pages 3317-3322
Journal: Food Chemistry - Volume 141, Issue 4, 15 December 2013, Pages 3317-3322
نویسندگان
Hongli Yang, Ying Mu, Hongtao Chen, Zhilong Xiu, Tiankui Yang,