Bioactive sesquiterpenoids from the solid culture of the edible mushroom Flammulina velutipes growing on cooked rice

► The edible mushroom Flammulina velutipes was cultivated on cooked rice. ► Eight new sesquiterpenes were purified and identified from its solid culture. ► The absolute configuration was assigned by X-ray, the Mosher and CD methods. ► Cuparene sesquiterpenes showed antimicrobial, cytotoxic and antioxidant activity. ► This is the first report of sterurane type sesquiterpene from this mushroom.