Purification of serine protease from mango (Mangifera Indica Cv. Chokanan) peel using an alcohol/salt aqueous two phase system

► Alcohol/salt based ATPS was used to purify serine protease from mango peel. ► Purification factor, yield, partition coefficient, and selectivity were optimised. ► Serine protease from mango peel is recovered with high purification factor (11.6) and yield (96.7%).