Physicochemical parameters that influence carotenoids bioaccessibility from a tomato juice

► A method was developed to evaluate impact of processing on lycopene bioaccessibility. ► This method focuses on the first step which is the release from the plant matrix. ► Lipid content appeared to be a limiting factor. ► Transfer of lycopene was influenced by the nature of the surfactant and temperature. ► In vitro models to assess bioaccessibility of carotenoids need to be revisited.