Multiple toxicity studies of trehalose in mice by intragastric administration

► Levels of 1.25 g/kg, 2.5 g/kg, and 5 g/kg of trehalose were conducted in mice. ► The sperm abnormality test was first used to detect reproductive toxicity. ► Bone marrow cell micronucleus test and haematology study were all performed. ► Organ coefficients and weight changes observing were designed in mice.