Non-targeted metabolomic approach reveals urinary metabolites linked to steroid biosynthesis pathway after ingestion of citrus juice

► Metabolomics is a powerful tool for identification of metabolites associated with dietary intake. ► Four urinary endocrine metabolites were identified as significant after citrus juice intake. ► The metabolites after 4 days of citrus juice intake belonged to the steroid pathway. ► This metabolomics approach provides mechanistic support to previous targeted studies.