Integrated biocatalytic process for trehalose production and separation from rice hydrolysate using a bioreactor system

► Our recombinant trehalose synthase (PTTS) was successfully produced in bioreactor system. ► Integrated enzymatic process to produce valuable products from rice hydrolysate in one step. ► Three bio-treatments were developed to simplify the separation and purification of trehalose. ► An efficient purification procedure for high purity trehalose production.