Heat stability of agaritine in water extracts from Agaricus blazei and other edible fungi, and removal of agaritine by ethanol fractionation

► Agaricus blazei and Agaricus bisporus contain agaritine which is a carcinogen. ► Agaritine contained in Agaricus water extracts is partially heat stable. ► Removal of agaritine from water extracts is carried out by ethanol fractionation. ► Analytical system (HPLC-MS) to quantify agaritine in mushrooms was established.