In vivo antitumoural activity and composition of an oil extract of Brazilian propolis

► Ethanolic and oil propolis extracts were evaluated in vivo in the Sarcoma 180 model. ► Toxicological aspects related to propolis extracts treatment were performed. ► The oil extract was as effective as the ethanolic extract at inhibiting tumour growth. ► Propolis reduced tumour growth with the same ratio as 5-FU but with less side effects. ► LC-MS/MS identified phenolic acids and flavonoids in oil propolis extract fractions.