Towards a quantitative application of real-time PCR technique for fish DNA detection in feedstuffs

► Develop of qPCR methods for fish DNA detection and quantification in feedstuffs. ► Design of primers and a Taqman probe to amplify the fish mitochondrial 12S rRNA. ► Standard curve approach on serial dilution of DNA from a reference fish flour. ► Specificity and sensitivity: forty different samples and LOD of 0.2 pg of fish DNA. ► Quantification of fishmeal in feed home made samples by ΔCT method.