Enzyme inactivation kinetics: Coupled effects of temperature and moisture content

► We investigate enzyme inactivation across a broad temperature-moisture content range. ► The inactivation is described with a two-step inactivation mechanism model. ► This model can accurately describe the experimental results. ► The model can also describe the transition of the determining step on inactivation. ► This approach is a solid basis in the investigation and optimisation of drying of enzymes.