Extraction and on-line concentration of flavonoids in Brassica oleracea by capillary electrophoresis using large volume sample stacking

► Demonstrated the ability of CE-UV-vis to quantify for flavonoids in broccoli. ► In-capillary concentration method to improve sensitivity. ► Successfully applied to the quantification of flavonoids in real extracts. ► The CE results were validated by HPLC analysis.