Optimisation and validation of a specific analytical method for the determination of thiram residues in fruits and vegetables by LC-MS/MS

► Specific analytical methodology is developed for determination of dithiocarbamate fungicide thiram in fruits and vegetables. ► The method is based on LC coupled to tandem MS. ► Thiram degradation along sample treatment is minimised, adding specific reagents and operating at around 5 °C. ► The excellent sensitivity and selectivity of the method allows monitoring for compliance of MRLs, with LOQ of 0.01 mg/kg. ► The acquisition of several SRM transitions allows the reliable confirmation of thiram in the samples at very low levels.